Generation of primary hepatocyte microarrays by piezoelectric printing

Zarowna-Dabrowska, Alicja, McKenna, Ekaterina O., Schütte, Maaike E., Glidle, Andrew, Chen, Li, Cuestas-Ayllon, Carlos, Marshall, Damian, Pitt, Andrew, Dawson, Martin D, Gu, Erdan, Cooper, Jon M. and Yin, Huabing (2012). Generation of primary hepatocyte microarrays by piezoelectric printing. Colloids and Surface B: Biointerfaces, 89 (1), pp. 126-132.


We demonstrate a single-step method for the generation of collagen and poly-l-Lysine (PLL) micropatterns on a poly(ethylene glycol) (PEG) functionalized glass surface for cell based assays. The method involves establishing a reliable silanization method to create an effective non-adhesive PEG layer on glass that inhibits cell attachment, followed by the spotting of collagen or PLL solutions using non-contact piezoelectric printing. We show for the first time that the spotted protein micropatterns remain stable on the PEG surface even after extensive washing, thus significantly simplifying protein pattern formation. We found that adherence and spreading of NIH-3T3 fibroblasts was confined to PLL and collagen areas of the micropatterns. In contrast, primary rat hepatocytes adhered and spread only on collagen micropatterns, where they formed uniform, well defined functionally active cell arrays. The differing affinity of hepatocytes and NIH-3T3 fibroblasts for collagen and PLL patterns was used to develop a simple technique for creating a co-culture of the two cell types. This has the potential to form structured arrays that mimic the in vivo hepatic environment and is easily integrated within a miniaturized analytical platform for developing high throughput toxicity analysis in vitro.

Publication DOI:
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Additional Information: Copyright © 2011 Elsevier B.V. All rights reserved.
Uncontrolled Keywords: animals,hepatocytes,mice,NIH 3T3 cells,Biotechnology,Colloid and Surface Chemistry,Physical and Theoretical Chemistry,Surfaces and Interfaces
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2012-01-01
Authors: Zarowna-Dabrowska, Alicja
McKenna, Ekaterina O.
Schütte, Maaike E.
Glidle, Andrew
Chen, Li
Cuestas-Ayllon, Carlos
Marshall, Damian
Pitt, Andrew ( 0000-0003-3619-6503)
Dawson, Martin D
Gu, Erdan
Cooper, Jon M.
Yin, Huabing


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