Methods to evaluate pesticide damage to the biomass of the soil microflora

Anderson, J.P.E., Armstrong, Richard A. and Smith, S.N. (1981). Methods to evaluate pesticide damage to the biomass of the soil microflora. Soil biology and biochemistry, 13 (2), pp. 149-153.


Respiratory methods to estimate the amount of C in the soil microbial biomass and the relative contributions of prokaryotes and eukaryotes in the biomass were used to evaluate the influence of pesticides on the soil microflora. Experiments were conducted with 5 and 50 micrograms per gram of three fungicides, captan, thiram and verdesan. At 5 micrograms per gram they caused significant decreases (40%) in the biomass; the organomercury fungicide verdesan also caused a shift from fungal to bacterial dominance. Within 8 days, biomass in captan- and thiram-amended soils had recovered to that of controls. Although the fungal to bacterial balance was restored in verdesan-amended soils, biomass recovery was not complete. At 50 micrograms per gram the fungicides caused long-term decreases in the biomass and altered the relative proportions of the bacterial and fungal populations. Verdesan had the greatest effect on soil microbial biomass and competition.

Publication DOI:
Divisions: Life & Health Sciences > Optometry
Life & Health Sciences > Ophthalmic Research Group
Life & Health Sciences
Life & Health Sciences > Biosciences
Uncontrolled Keywords: respiratory methods,soil microbial biomass,procaryotes,eucaryotes,pesticides,soil microflora
Published Date: 1981
Authors: Anderson, J.P.E.
Armstrong, Richard A. ( 0000-0002-5046-3199)
Smith, S.N.


Item under embargo.

Export / Share Citation


Additional statistics for this record