Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

Perry, A.L., Worthington, Tony, Hilton, Anthony, Lambert, Peter A., Stirling, A.J. and Elliott, T.S.J. (2003). Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD. FEMS Microbiology Letters, 228 (1), pp. 51-55.

Abstract

Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Publication DOI: https://doi.org/10.1016/S0378-1097(03)00720-1
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: molecular typing,propionibacterium acnes,random amplification of polymorphic DNA,Genetics,Molecular Biology,Applied Microbiology and Biotechnology,Microbiology
Full Text Link: http://onlineli ... 0720-1/abstract
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2003-11-07
Authors: Perry, A.L.
Worthington, Tony ( 0000-0002-1906-3357)
Hilton, Anthony ( 0000-0001-8025-5270)
Lambert, Peter A. ( 0000-0002-8243-2741)
Stirling, A.J.
Elliott, T.S.J.

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