Antimicrobial efficacy of chlorhexidine digluconate alone and in combination with eucalyptus oil, tea tree oil and thymol against planktonic and biofilm cultures of Staphylococcus epidermidis

Karpanen, T.J., Worthington, Tony, Hendry, E.R., Conway, B.R. and Lambert, Peter A. (2008). Antimicrobial efficacy of chlorhexidine digluconate alone and in combination with eucalyptus oil, tea tree oil and thymol against planktonic and biofilm cultures of Staphylococcus epidermidis. Journal of Antimicrobial Chemotherapy, 62 (5), pp. 1031-1036.

Abstract

Objectives Effective skin antisepsis and disinfection of medical devices are key factors in preventing many healthcare-acquired infections associated with skin microorganisms, particularly Staphylococcus epidermidis. The aim of this study was to investigate the antimicrobial efficacy of chlorhexidine digluconate (CHG), a widely used antiseptic in clinical practice, alone and in combination with tea tree oil (TTO), eucalyptus oil (EO) and thymol against planktonic and biofilm cultures of S. epidermidis. Methods Antimicrobial susceptibility assays against S. epidermidis in a suspension and in a biofilm mode of growth were performed with broth microdilution and ATP bioluminescence methods, respectively. Synergy of antimicrobial agents was evaluated with the chequerboard method. Results CHG exhibited antimicrobial activity against S. epidermidis in both suspension and biofilm (MIC 2–8 mg/L). Of the essential oils thymol exhibited the greatest antimicrobial efficacy (0.5–4 g/L) against S. epidermidis in suspension and biofilm followed by TTO (2–16 g/L) and EO (4–64 g/L). MICs of CHG and EO were reduced against S. epidermidis biofilm when in combination (MIC of 8 reduced to 0.25–1 mg/L and MIC of 32–64 reduced to 4 g/L for CHG and EO, respectively). Furthermore, the combination of EO with CHG demonstrated synergistic activity against S. epidermidis biofilm with a fractional inhibitory concentration index of <0.5. Conclusions The results from this study suggest that there may be a role for essential oils, in particular EO, for improved skin antisepsis when combined with CHG.

Publication DOI: https://doi.org/10.1093/jac/dkn325
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Applied Health Research Group
Life & Health Sciences > Pharmacy
Additional Information: This is an electronic version of an article published in Karpanen, T.J.; Worthington, Tony; Hendry, E.R.; Conway, B.R. and Lambert, Peter A. (2008). Antimicrobial efficacy of chlorhexidine digluconate alone and in combination with eucalyptus oil, tea tree oil and thymol against planktonic and biofilm cultures of Staphylococcus epidermidis. Journal of Antimicrobial Chemotherapy, 62 (5), pp. 1031-1036. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
Uncontrolled Keywords: essential oils,chlorhexidine,synergism,skin antisepsis,antimicrobial activity,Infectious Diseases,Pharmacology (medical),Pharmacology
Full Text Link: http://jac.oxfordjournals.org/content/62/5/1031.abstract
Related URLs: http://www.scopus.com/inward/record.url?scp=54549105525&partnerID=8YFLogxK (Scopus URL)
Published Date: 2008-11
Authors: Karpanen, T.J.
Worthington, Tony
Hendry, E.R.
Conway, B.R.
Lambert, Peter A. ( 0000-0002-8243-2741)

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