The role of tissue transglutaminase in 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity in differentiated human SH-SY5Y neuroblastoma cells

Beck, Katy E., De Girolamo, Luigi A., Griffin, Martin and Billett, E. Ellen The role of tissue transglutaminase in 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity in differentiated human SH-SY5Y neuroblastoma cells. Neuroscience Letters, 405 (1-2), pp. 46-51.

Abstract

Tissue transglutaminase (TG2) can induce post-translational modification of proteins, resulting in protein cross-linking or incorporation of polyamines into substrates, and can also function as a signal transducing G protein. The role of TG2 in the formation of insoluble cross-links has led to its implication in some neurodegenerative conditions. Exposure of pre-differentiated SH-SY5Y cells to the Parkinsonian neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+) resulted in significant dose-dependent reductions in TG2 protein levels, measured by probing Western blots with a TG2-specific antibody. Transglutaminase (TG) transamidating activity, on the other hand, monitored by incorporation of a polyamine pseudo-substrate into cellular proteins, was increased. Inhibitors of TG (putrescine) and TG2 (R283) exacerbated MPP+ toxicity, suggesting that activation of TG2 may promote a survival response in this toxicity paradigm.

Publication DOI: https://doi.org/10.1016/j.neulet.2006.06.061
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Applied Health Research Group
Life & Health Sciences > Biomedical Sciences research group
Aston University (General)
Uncontrolled Keywords: Parkinson's disease,MPP+,tissue transglutaminase,SH-SY5Y human neuroblastoma,cell viability
Full Text Link: http://www.scopus.com/inward/record.url?scp=33746472503&partnerID=8YFLogxK
Related URLs:
Authors: Beck, Katy E.
De Girolamo, Luigi A.
Griffin, Martin
Billett, E. Ellen

Download

[img]

Version: Published Version

Export / Share Citation


Statistics

Additional statistics for this record