Identification of a novel inositol phosphate recognition site: specific [3H]inositol hexakisphosphate binding to brain regions and cerebellar membranes

Hawkins, Phillip T., Reynolds, D.J.M., Poyner, David R. and Hanley, Michael R. (1990). Identification of a novel inositol phosphate recognition site: specific [3H]inositol hexakisphosphate binding to brain regions and cerebellar membranes. Biochemical and Biophysical Research Communications, 167 (2), pp. 819-827.

Abstract

[3H]Inositol hexakisphosphate (InsP6) binds with a heterogeneous distribution to frozen sections of unfixed rat brain and is displaced by unlabelled InsP6. The pattern of binding correlates with binding to neuronal cell bodies. [3H]InsP6 binding to cerebellar membranes has been further characterised, is reversible, and saturable, and exhibits high specificity for inositol polyphosphates. The IC50 for competition by unlabelled InsP6 is approximately 100nM, whereas inositol 1,3,4,5,6 pentakisphosphate (Ins(13456)P5), inositol 1,3,4,5 tetrakisphosphate (Ins(1345)P4), and inositol 1,4,5 trisphosphate (Ins(145)P3) bind with an affinity at least one order of magnitude lower. [3H]InsP6 binding is clearly distinct from previously characterised Ins(145)P3 (ref. 1, 2) and Ins(1345)P4 (ref. 3) binding, both in terms of pharmacology and brain distribution.

Publication DOI: https://doi.org/10.1016/0006-291X(90)92099-L
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Uncontrolled Keywords: animals,autoradiography,binding sites,brain,brain stem,cattle,cell membrane,cerebellum chromatography,high pressure liquid,inositol phosphates,kinetics,phytic acid,rats,tritium
Published Date: 1990-03-16
Authors: Hawkins, Phillip T.
Reynolds, D.J.M.
Poyner, David R. ( 0000-0003-1590-112X)
Hanley, Michael R.

Download

Item under embargo.

Export / Share Citation


Statistics

Additional statistics for this record