Stimulation of adenylate cyclase by amylin in CHO-K1 cells

D'Santos, C.S.; Gatti, A.; Poyner, David R. and Hanley, Michael R. (1992). Stimulation of adenylate cyclase by amylin in CHO-K1 cells. Molecular Pharmacology, 41 (5), pp. 894-899.

Abstract

The CHO-K1 cell line responds to the peptide amylin by a rapid elevation of cAMP. The related peptide calcitonin gene-related peptide (CGRP) is 100 times less potent at stimulating adenylate cyclase than is amylin. The actions of amylin at this receptor are concentration dependent and not antagonized by the CGRP antagonist CGRP-(8-37). Although these cells have receptors for calcitonin, amylin is unable to take part in any high affinity interaction with these receptors, as assessed by radioligand binding. The CHO-K1 cell line has receptors for amylin that are distinct from those for calcitonin and CGRP.

Divisions: Life & Health Sciences > Pharmacy
Uncontrolled Keywords: 1-Methyl-3-isobutylxanthine,adenylate,cyclase,amyloid,animals,CHO,cells,calcitonin,gene-related peptide,cricetinae cyclic,AMP,dose-response relationship,drug,enzyme activation,humans kinetics receptors,calcitonin receptors,cell surface
Published Date: 1992-05-01

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