Receptor regulation of phosphoinositide 3-hydroxykinase in the NG115-401L-C3 neuronal cell line: stimulation by insulin-like growth factor-I

Poyner, David R., Hanley, Michael R., Jackson, T.R. and Hawkins, Phillip T. (1993). Receptor regulation of phosphoinositide 3-hydroxykinase in the NG115-401L-C3 neuronal cell line: stimulation by insulin-like growth factor-I. Biochemical Journal, 290 , pp. 901-905.

Abstract

The activation of phosphoinositide 3-hydroxykinase (P13K) is currently believed to represent the critical regulatory event which leads to the production of a novel intracellular signal. We have examined the control of this pathway by a number of cell-surface receptors in NG115-401L-C3 neuronal cells. Insulin-like growth factor-I stimulated the accumulation of 3-phosphorylated inositol lipids in intact cells and the appearance of P13K in antiphosphotyrosine-antibody-directed immunoprecipitates prepared from lysed cells, suggesting that P13K had been activated by a mechanism involving a protein tyrosine kinase. In contrast, P13K in these cells was not regulated by a variety of G-protein-coupled receptors, nerve growth factor acting via a low affinity receptor, or receptors for transforming growth factor-beta and interleukin-1. The receptor-specificity of P13K activation in these cells places significant constraints on the possible physiological function(s) of this pathway.

Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Uncontrolled Keywords: 1-Phosphatidylinositol,3-Kinase,animals,cell line,cell membrane,enzyme activation,immunosorbent techniques,insulin-like growth factor,I kinetics,nerve growth factors,neurons,phosphatidylinositol phosphates,phosphatidylinositols,phosphotransferases receptors,cCell surface receptors
Full Text Link: http://www.biochemj.org/bj/290/bj2900901.htm
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Published Date: 1993-04-21
Authors: Poyner, David R. ( 0000-0003-1590-112X)
Hanley, Michael R.
Jackson, T.R.
Hawkins, Phillip T.

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