Selective recognition of cyclic GMP using a fluorescence-based molecularly imprinted polymer

Thanh, Nguyen Thi Kim, Rathbone, Daniel L., Billington, David C. and Hartell, Nicholas A. (2002). Selective recognition of cyclic GMP using a fluorescence-based molecularly imprinted polymer. Analytical letters, 35 (15), pp. 2499-2509.


Guanosine 3′,5′-cyclic monophosphate (cGMP) plays a role as a second messenger in many different biological systems. Given the ubiquitous nature of cGMP, a simple method of detecting cGMP is of interest. To that end a fluorescent polymer with recognition sites for cGMP has been prepared. Its selectivity and sensitivity were investigated and a dose-dependant decrease in fluorescence of the polymer in the presence of cGMP was observed. In contrast, virtually no effect was detected upon application of the structurally similar molecules, guanosine 5′-monophosphate (GMP) and adenosine 3′,5′-cyclic monophosphate (cAMP), thus demonstrating the high selectivity of this polymer. The association constant for the binding of cGMP to the imprinted polymer was determined in order of 3 × 10 5 M -1. A fluorescent, molecularly imprinted polymer that selectively recognises cGMP may have a useful application as a fluorescent chemosensor for cGMP detection in biological samples.

Publication DOI:
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Uncontrolled Keywords: cAMP,cGMP,fluorescent monomer,molecular recognition,molecularly imprinted polymer (MIP),Analytical Chemistry,Biochemistry, Genetics and Molecular Biology(all),Biochemistry
Full Text Link: ... e=15&spage=2499
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2002-12
Authors: Thanh, Nguyen Thi Kim
Rathbone, Daniel L. ( 0000-0003-4732-4662)
Billington, David C.
Hartell, Nicholas A.

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