Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor

Karow, J, Hudson, K R, Hall, M A, Vernallis, Ann, Taylor, J A, Gossler, A and Heath, J K (1996). Mediation of interleukin-11-dependent biological responses by a soluble form of the interleukin-11 receptor. Biochemical Journal, 318 (2), pp. 489-95.

Abstract

Interleukin-11 (IL-11) is a polyfunctional cytokine whose biological actions require a specific IL-11 receptor (IL-11R) and the transmembrane transducer gp130. Here we report the production of a soluble form of the murine IL-11R and demonstrate that it interacts with IL-11 ligand with high affinity. The affinity of IL-11 alone for gp130 is below the level of detection, but a complex of IL-11 and soluble IL-11R interacts with gp130 with high affinity. The addition of soluble IL-11R potentiates the effects of exogenous IL-11 in cells that are normally responsive to IL-11. A biological response to IL-11 can be reconstituted in BAF cells transfected with gp130 by addition of IL-11 and soluble IL-11R. These findings show that the cytoplasmic domain of the IL-11R is not required for the biological effects of IL-11 and that a complex of IL-11 and IL-11R mediates signalling by association with gp130.

Publication DOI: https://doi.org/10.1042/bj3180489
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Uncontrolled Keywords: Animals,Antigens, CD,B-Lymphocytes,Cell Division,Cell Line,Cytokine Receptor gp130,Electrophoresis, Polyacrylamide Gel,Genetic Vectors,Humans,Interleukin-11,Interleukin-11 Receptor alpha Subunit,Kinetics,Membrane Glycoproteins,Mice,Polymerase Chain Reaction,Receptors, Interleukin,Receptors, Interleukin-11,Recombinant Proteins,Signal Transduction,Transfection
Full Text Link: http://www.ncbi ... /pubmed/8809037
Related URLs: http://www.bioc ... /318/2/489.long (Publisher URL)
Published Date: 1996-09-01
Authors: Karow, J
Hudson, K R
Hall, M A
Vernallis, Ann
Taylor, J A
Gossler, A
Heath, J K

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