Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein

Teo, Alvin C. K., Lee, Sarah C., Pollock, Naomi L., Stroud, Zoe, Hall, Stephen, Thakker, Alpesh, Pitt, Andrew R., Dafforn, Timothy R., Spickett, Corinne M. and Roper, David I. (2019). Analysis of SMALP co-extracted phospholipids shows distinct membrane environments for three classes of bacterial membrane protein. Scientific Reports, 9 (1),

Abstract

Biological characterisation of membrane proteins lags behind that of soluble proteins. This reflects issues with the traditional use of detergents for extraction, as the surrounding lipids are generally lost, with adverse structural and functional consequences. In contrast, styrene maleic acid (SMA) copolymers offer a detergent-free method for biological membrane solubilisation to produce SMA-lipid particles (SMALPs) containing membrane proteins together with their surrounding lipid environment. We report the development of a reverse-phase LC-MS/MS method for bacterial phospholipids and the first comparison of the profiles of SMALP co-extracted phospholipids from three exemplar bacterial membrane proteins with different topographies: FtsA (associated membrane protein), ZipA (single transmembrane helix), and PgpB (integral membrane protein). The data showed that while SMA treatment per se did not preferentially extract specific phospholipids from the membrane, SMALP-extracted ZipA showed an enrichment in phosphatidylethanolamines and depletion in cardiolipins compared to the bulk membrane lipid. Comparison of the phospholipid profiles of the 3 SMALP-extracted proteins revealed distinct lipid compositions for each protein: ZipA and PgpB were similar, but in FtsA samples longer chain phosphatidylglycerols and phosphatidylethanolamines were more abundant. This method offers novel information on the phospholipid interactions of these membrane proteins.

Publication DOI: https://doi.org/10.1038/s41598-018-37962-0
Dataset DOI: https://doi.org/10.17036/researchdata.aston.ac.uk.00000393
Divisions: Life & Health Sciences
Life & Health Sciences > Pharmacy
Life & Health Sciences > Biosciences
Additional Information: © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Uncontrolled Keywords: General
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Related URLs: http://www.natu ... 598-018-37962-0 (Publisher URL)
http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Accepted Date: 2019-12-12
Published Online Date: 2019-02-12
Published Date: 2019-02-12
Authors: Teo, Alvin C. K.
Lee, Sarah C.
Pollock, Naomi L.
Stroud, Zoe
Hall, Stephen
Thakker, Alpesh
Pitt, Andrew R. ( 0000-0003-3619-6503)
Dafforn, Timothy R.
Spickett, Corinne M. ( 0000-0003-4054-9279)
Roper, David I.

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