A real time immunoassay in alumina membranes

Álvarez, J., Sola, L., Cretich, M., Swann, M. J., Gylfasson, K. B., Volden, T., Chiari, M. and Hill, D. (2014). A real time immunoassay in alumina membranes. Proceedings of IEEE Sensors, 2014-D (Decemb), pp. 1760-1763.

Abstract

To date, photonic biosensing with porous membranes has produced slow responses and long sensing times, due to the narrow (less than 100 nm) closed end pores of the membranes used. Recently, polarimetry was used to demonstrate analyte flow through, and real time biosensing in, free-standing porous alumina membranes. Here, we demonstrate how an improved functionalization technology, has for the first time enabled a real-time immunoassay within a porous membrane with a total assay time below one hour. With the new approach, we show a noise floor for individual biosensing measurements of 3.7 ng/ml (25 pM), and a bulk refractive index detection limit of 5×10-6 RIU, with a standard deviation of less than 5%. The membranes, with their 200 nm pore diameter enabling targeted delivering of analytes to bioreceptors immobilized on the pore walls, therefore provide a route towards rapid and low cost realtime opto-fluidic biosensors for small sample volumes.

Publication DOI: https://doi.org/10.1109/ICSENS.2014.6985365
Divisions: Engineering & Applied Sciences > Institute of Photonics
Uncontrolled Keywords: Copolymer,Form birefringence,Optical biosensing,Polarimetry,Porous alumina,Quantum dots,Electrical and Electronic Engineering
Full Text Link: http://kth.diva ... 7282/FULLTEXT01
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
https://ieeexpl ... r=6985365&tag=1 (Publisher URL)
Published Date: 2014-12-15
Authors: Álvarez, J.
Sola, L.
Cretich, M.
Swann, M. J.
Gylfasson, K. B.
Volden, T.
Chiari, M.
Hill, D. ( 0000-0003-3510-7783)

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