Importance of Propionibacterium acnes hemolytic activity in human intervertebral discs:A microbiological study

Capoor, Manu N, Ruzicka, Filip, Sandhu, Gurpreet, Rollason, Jess, Mavrommatis, Konstantinos, Ahmed, Fahad S, Schmitz, Jonathan E, Raz, Assaf, Brüggemann, Holger, Lambert, Peter A, Fischetti, Vincent A and Slaby, Ondrej (2018). Importance of Propionibacterium acnes hemolytic activity in human intervertebral discs:A microbiological study. PLoS ONE, 13 (11),

Abstract

Most patients with chronic lower back pain (CLBP) exhibit degenerative disc disease. Disc specimens obtained during initial therapeutic discectomies are often infected/colonized with Propionibacterium acnes, a Gram-positive commensal of the human skin. Although pain associated with infection is typically ascribed to the body's inflammatory response, the Gram-positive bacterium Staphylococcus aureus was recently observed to directly activate nociceptors by secreting pore-forming α-hemolysins that disrupt neuronal cell membranes. The hemolytic activity of P. acnes in cultured disc specimens obtained during routine therapeutic discectomies was assessed through incubation on sheep-blood agar. The β-hemolysis pattern displayed by P. acnes on sheep-blood agar was variable and phylogroup-dependent. Their molecular phylogroups were correlated with their hemolytic patterns. Our findings raise the possibility that pore-forming proteins contribute to the pathogenesis and/or symptomology of chronic P. acnes disc infections and CLBP, at least in a subset of cases.

Publication DOI: https://doi.org/10.1371/journal.pone.0208144
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Additional Information: © 2018 Capoor et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Related URLs: https://journal ... al.pone.0208144 (Publisher URL)
Published Date: 2018-11-29
Authors: Capoor, Manu N
Ruzicka, Filip
Sandhu, Gurpreet
Rollason, Jess
Mavrommatis, Konstantinos
Ahmed, Fahad S
Schmitz, Jonathan E
Raz, Assaf
Brüggemann, Holger
Lambert, Peter A ( 0000-0002-8243-2741)
Fischetti, Vincent A
Slaby, Ondrej

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