Inositol polyphosphate-mediated iron transport in Pseudomonas aeruginosa

Hirst, Peter H., Riley, A.M., Mills, S.J., Spiers, Ian D., Poyner, David R., Freeman, Sally, Potter, B.V. and Smith, Anthony W. (1999). Inositol polyphosphate-mediated iron transport in Pseudomonas aeruginosa. Journal of Applied Microbiology, 86 (3), pp. 537-543.


It has previously been shown that myo-inositol hexakisphosphate (myo- InsP6) mediates iron transport into Pseudomonas aeruginosa and overcomes iron-dependent growth inhibition. In this study, the iron transport properties of myo-inositol trisphosphate and tetrakisphosphate regio-isomers were studied. Pseudomonas aeruginosa accumulated iron (III) at similar rates whether complexed with myo-Ins(1,2,3)P3 or myo-InsP6. Iron accumulation from other compounds, notably D/L myo-Ins(1,2,4,5)P4 and another inositol trisphosphate regio-isomer, D-myo-Ins(1,4,5)P3, was dramatically increased. Iron transport profiles from myo-InsP6 into mutants lacking the outer membrane porins oprF, oprD and oprP were similar to the wild-type, indicating that these porins are not involved in the transport process. The rates of reduction of iron (III) to iron (II) complexed to any of the compounds by a Ps. aeruginosa cell lysate were similar, suggesting that a reductive mechanism is not the rate-determining step.

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Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Uncontrolled Keywords: biological transport,inositol,phosphates,iron,iron radioisotopes,NADH,NADPH,oxidoreductases,porins,pseudomonas aeruginosa
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Published Date: 1999-03-27
Authors: Hirst, Peter H.
Riley, A.M.
Mills, S.J.
Spiers, Ian D.
Poyner, David R. ( 0000-0003-1590-112X)
Freeman, Sally
Potter, B.V.
Smith, Anthony W.

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