Development and Characterisation of a Human Chronic Skin Wound Cell Line:Towards an Alternative for Animal Experimentation

Caley, Matthew, Wall, Ivan, Peake, Matthew, Kipling, David, Giles, Peter, Thomas, David and Stephens, Phil (2018). Development and Characterisation of a Human Chronic Skin Wound Cell Line:Towards an Alternative for Animal Experimentation. International Journal of Molecular Sciences, 19 (4),

Abstract

Background: Chronic skin wounds are a growing financial burden for healthcare providers, causing discomfort/immobility to patients. Whilst animal chronic wound models have been developed to allow for mechanistic studies and to develop/test potential therapies, such systems are not good representations of the human chronic wound state. As an alternative, human chronic wound fibroblasts (CWFs) have permitted an insight into the dysfunctional cellular mechanisms that are associated with these wounds. However, such cells strains have a limited replicative lifespan and therefore a limited reproducibility/usefulness. Objectives: To develop/characterise immortalised cell lines of CWF and patient-matched normal fibroblasts (NFs). Methods and Results: Immortalisation with human telomerase resulted in both CWF and NF proliferating well beyond their replicative senescence end-point (respective cell strains senesced as normal). Gene expression analysis demonstrated that, whilst proliferation-associated genes were up-regulated in the cell lines (as would be expected), the immortalisation process did not significantly affect the disease-specific genotype. Immortalised CWF (as compared to NF) also retained a distinct impairment in their wound repopulation potential (in line with CWF cell strains). Conclusions: These novel CWF cell lines are a credible animal alternative and could be a valuable research tool for understanding both the aetiology of chronic skin wounds and for therapeutic pre-screening.

Publication DOI: https://doi.org/10.3390/ijms19041001
Divisions: Life & Health Sciences
Additional Information: © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Full Text Link:
Related URLs: http://www.mdpi ... -0067/19/4/1001 (Publisher URL)
Published Date: 2018-03-27
Authors: Caley, Matthew
Wall, Ivan ( 0000-0001-6294-8348)
Peake, Matthew
Kipling, David
Giles, Peter
Thomas, David
Stephens, Phil

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