Particulate material for purification of bio-macromolecules

Olander, Morten A. (2004). Particulate material for purification of bio-macromolecules. US6783962 B1.

Abstract

The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.

Publication DOI: US6783962 B1
Divisions: Engineering & Applied Sciences
Full Text Link: https://worldwi ... 6783962B1&KC=B1
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Published Date: 2004-08-31
Authors: Olander, Morten A.
Lihme, Allan O.F.
Hobley, Timothy J.
Simon, Marcos
Theodossiou, Irene ( 0000-0001-7068-4434)
Thomas, Owen R.T.

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