A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice

Litvinova, Karina S., Ahmad, Shakil, Wang, Keqing, Rafailov, Ilya E., Sokolovski, Sergei G., Zhang, Lin, Rafailov, Edik U. and Ahmed, Asif (2016). A pilot study using laser-based technique for non-invasive diagnostics of hypertensive conditions in mice. IN: SPIE BiOS, 2016, San Francisco, California, United States. 2016-02-29.

Abstract

Endothelial dysfunction is directly linked to preeclampsia, a maternal hypertensive condition that is life threating for both the mother and the baby. Epidemiological studies show that women with a history of pre-eclampsia have an elevated risk for cardiovascular disease. Here we report a new non-invasive diagnostic test for preeclampsia in mice that allows us to non-invasively assess the condition of the animals during the experiment and treatment in established models of preeclampsia. A laser-based multifunctional diagnostics system (LAKK-M) was chosen to carry out non-invasive analysis of multiple parameters. The device was used to simultaneously record the microcirculatory blood flow and oxygen saturation, as well as fluorescence levels of endogenous fluorophores. Preliminary experiments were conducted on adenoviral (Ad-)- mediated overexpression of sFlt-1 (Ad-sFlt-1) to mimic preeclampsialike symptoms in mice. The recorded data displayed the ability of the LAKK-M diagnostics device to detect significant differences in perfusion measurements between the control and Ad-sFlt-1 treatment. Preliminary results provide a potential avenue to employ these diagnostics technology to monitor and aid in maintaining control of live animal conditions throughout the experiment and treatment.

Publication DOI: https://doi.org/10.1117/12.2213026
Divisions: Engineering & Applied Sciences
Aston Medical School
Life & Health Sciences > Biosciences
Life & Health Sciences
Engineering & Applied Sciences > Electrical, electronic & power engineering
Engineering & Applied Sciences > Institute of Photonics
Additional Information: © (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).
Event Title: SPIE BiOS, 2016, San Francisco, California, United States
Event Type: Other
Event Dates: 2016-02-29
Full Text Link: http://proceedi ... 1117/12.2213026
Related URLs:
Published Date: 2016-02-29
Authors: Litvinova, Karina S.
Ahmad, Shakil ( 0000-0002-9294-0475)
Wang, Keqing
Rafailov, Ilya E.
Sokolovski, Sergei G.
Zhang, Lin ( 0000-0002-1691-5577)
Rafailov, Edik U. ( 0000-0002-4152-0120)
Ahmed, Asif

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