Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification

Alotaibi, Mohammed, Manayil, Jinesh C., Greenway, Gillian M., Haswell, Stephen J., Kelly, Stephen M., Lee, Adam F., Wilson, Karen and Kyriakou, Georgios (2017). Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification. Reaction Chemistry & Engineering ,

Abstract

Lipase immobilised on silica monoliths has been prepared and applied as biocatalytic continuous-flow microreactors for the transesterification of tributyrin as a model bio-oil component. Candida antarctica lipase was trapped within the pores of silica monoliths, and its successful immobilisation was demonstrated by the hydrolysis of 4-nitrophenyl butyrate to 4-nitrophenol. Lipase immobilised on silica monoliths was active for the transesterification of tributyrin at ambient temperature, with reactivity as a function of the methanol : tributyrin ratio, flow rate, temperature, and textural properties. Monoliths with a high surface area and large meso- and macropore channels enhanced the transesterification activity through improved molecule diffusion. The optimum immobilised lipase microreactor exhibited almost quantitative ester production for >100 h at 30 °C without deactivation

Publication DOI: https://doi.org/10.1039/C7RE00162B
Divisions: Engineering & Applied Sciences
Engineering & Applied Sciences > European Bioenergy Research Institute (EBRI)
Engineering & Applied Sciences > Chemical engineering & applied chemistry
Additional Information: © Royal Society of Chemistry, 2017. Lipase immobilised on silica monoliths as continuous-flow microreactors for triglyceride transesterification Alotaibi, M., Manayil, J. C., Greenway, G. M., Haswell, S. J., Kelly, S. M., Lee, A. F., Wilson, K. & Kyriakou, G. 18 Dec 2017 In : Reaction Chemistry & Engineering. http://doi.org/10.1039/C7RE00162B
Full Text Link: http://xlink.rs ... ?DOI=C7RE00162B
Related URLs: https://www.sco ... 30ab1b423af66f1 (Scopus URL)
Published Date: 2017-12-18
Authors: Alotaibi, Mohammed
Manayil, Jinesh C.
Greenway, Gillian M.
Haswell, Stephen J.
Kelly, Stephen M.
Lee, Adam F. ( 0000-0002-2153-1391)
Wilson, Karen ( 0000-0003-4873-708X)
Kyriakou, Georgios

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