Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

Dimov, Nikolay, Kastner, Elisabeth, Hussain, Maryam, Perrie, Yvonne and Szita, Nicolas (2017). Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system. Scientific Reports, 7 (1),

Abstract

Liposomes are lipid based bilayer vesicles that can encapsulate, deliver and release low-soluble drugs and small molecules to a specific target site in the body. They are currently exploited in several nanomedicine formulations. However, their development and application is still limited by expensive and time-consuming process development and production methods. Therefore, to exploit these systems more effectively and support the rapid translation of new liposomal nanomedicines from bench to bedside, new cost-effective and scalable production methods are needed. We present a continuous process flow system for the preparation, modification and purification of liposomes which offers lab-on-chip scale production. The system was evaluated for a range of small vesicles (below 300 nm) varying in lipid composition, size and charge; it offers effective and rapid nanomedicine purification with high lipid recovery (> 98%) combined with effective removal of non-entrapped drug (propofol >95% reduction of non-entrapped drug present) or protein (ovalbumin >90% reduction of OVA present) and organic solvent (ethanol >95% reduction) in less than 4 minutes. The key advantages of using this bench-top, rapid, process development tool are the flexible operating conditions, interchangeable membranes and scalable high-throughput yields, thereby offering simultaneous manufacturing and purification of nanoparticles with tailored surface attributes.

Publication DOI: https://doi.org/10.1038/s41598-017-11533-1
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Additional Information: Copyright: The authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Funding: EPSRC Centre for Innovative Manufacturing in Emergent Macromolecular Therapies and Aston University. BBSRC (BB/L000997/1) and the European Research Area initiative on industrial biotechnology (ERA-IB; third joint call).
Uncontrolled Keywords: Drug delivery ,Microfluidics ,Biomedical engineering,Drug development,General
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2017-09-21
Authors: Dimov, Nikolay
Kastner, Elisabeth
Hussain, Maryam
Perrie, Yvonne
Szita, Nicolas

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