Characterisation of the porcine eyeball as an in-vitro model for dry eye

Menduni, Francesco, Davies, Leon N, Madrid-Costa, David, Fratini, Antonio and Wolffsohn, James S. (2017). Characterisation of the porcine eyeball as an in-vitro model for dry eye. Contact Lens and Anterior Eye, 41 (1), pp. 13-17.

Abstract

Purpose: To characterise the anatomical parameters of the porcine eye for potentially using it as a laboratory model of dry eye. Methods: Anterior chamber depth and angle, corneal curvature, shortest and longest diameter, endothelial cell density, and pachymetry were measured in sixty freshly enucleated porcine eyeballs. Results: Corneal steepest meridian was 7.85 ± 0.32 mm, corneal flattest meridian was 8.28 ± 0.32 mm, shortest corneal diameter was 12.69 ± 0.58 mm, longest corneal diameter was 14.88 ± 0.66 mm and central corneal ultrasonic pachymetry was 1009 ± 1μm. Anterior chamber angle was 28.83 ± 4.16°, anterior chamber depth was 1.77 ± 0.27 mm, and central corneal thickness measured using OCT was 1248 ± 144μm. Corneal endothelial cell density was 3250 ± 172 cells/mm2. Conclusions: Combining different clinical techniques produced a pool of reproducible data on the porcine eye anatomy, which can be used by researchers to assess the viability of using the porcine eye as an in-vitro/ex-vivo model for dry eye. Due to the similar morphology with the human eye, porcine eyeballs may represent a useful and cost effective model to individually study important key factors in the development of dry eye, such as environmental and mechanical stresses.

Publication DOI: https://doi.org/10.1016/j.clae.2017.09.003
Divisions: Life & Health Sciences > Optometry
Life & Health Sciences > Ophthalmic Research Group
Life & Health Sciences
Additional Information: © 2017 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/. Funding: European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 642760.
Uncontrolled Keywords: Porcine eye ,Dry eye ,Confocal microscopy ,Optical coherence tomography
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Related URLs: http://www.scie ... 2473?via%3Dihub (Publisher URL)
Published Date: 2017-10-03
Published Online Date: 2017-10-03
Authors: Menduni, Francesco
Davies, Leon N ( 0000-0002-1554-0566)
Madrid-Costa, David
Fratini, Antonio ( 0000-0001-8894-461X)
Wolffsohn, James S. ( 0000-0003-4673-8927)

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