Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Cartwright, Stephanie, Bill, Roslyn M., Bui, Sy Tien, Van, Hieu Tran and Nguyen, Hung Minh (2017). Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris. BMC Research Notes, 10 ,

Abstract

Objective: Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. Results: This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni2+ affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study.

Publication DOI: https://doi.org/10.1186/s13104-017-2692-8
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Biomedical Sciences research group
Additional Information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords: HDAg,hepatitis delta virus,pichia pastoris,protein expression,Medicine(all),Biochemistry, Genetics and Molecular Biology(all)
Full Text Link: http://www.scopus.com/inward/record.url?scp=85026207281&partnerID=8YFLogxK
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Published Date: 2017-07-27
Authors: Cartwright, Stephanie
Bill, Roslyn M.
Bui, Sy Tien
Van, Hieu Tran
Nguyen, Hung Minh

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