Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris

Cartwright, Stephanie; Bill, Roslyn M.; Bui, Sy Tien; Van, Hieu Tran and Nguyen, Hung Minh Rapid expression and purification of the hepatitis delta virus antigen using the methylotropic yeast Pichia pastoris. BMC Research Notes, 10 ,

Abstract

Objective: Patients with dual hepatitis B (HBV) and hepatitis D (HDV) virus infection are at an increased risk of progression to liver cirrhosis and hepatocellular carcinoma than patients with a single viral infection. Treatment of viral hepatitis due to dual HBV/HDV infection represents a challenge. Currently there is no vaccine against HDV. Recombinant production of HDV antigen (HDAg) is the first step towards a potential vaccine candidate and the development of assays for HDV detection. Results: This study demonstrates the expression of one HDAg isoform, S-HDAg, in Pichia pastoris. A recombinant vector carrying a tagged gene encoding S-HDAg under the control of the methanol-inducible promoter AOX1 was designed and integrated into P. pastoris X33. The protein, which was purified using a Ni2+ affinity column and eluted at 100-150 mM imidazole, has potential as a recombinant antigen for further study.

Publication DOI: https://doi.org/10.1186/s13104-017-2692-8
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Biomedical Sciences research group
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Additional Information: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords: HDAg,hepatitis delta virus,pichia pastoris,protein expression,Medicine(all),Biochemistry, Genetics and Molecular Biology(all)

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