Reconstitution of membrane proteins:a GPCR as an example

Goddard, Alan D., Dijkman, Patricia M., Adamson, Roslin J., dos Reis, Rosana Inácio and Watts, Anthony (2015). Reconstitution of membrane proteins:a GPCR as an example. Methods in Enzymology, 556 , pp. 405-424.

Abstract

Membrane proteins are the gatekeepers to the cell and are essential to the function of all cells, controlling the flow of molecules and information across the cell membrane. Much effort has been put into the development of systems for studying membrane proteins in simplified environments that nevertheless mimic their native lipid environment. After isolation and production of purified membrane proteins in detergent, it is often necessary to reconstitute them into a lipid structure such as liposome, nanodisc, or lipodisq. Each of these has the advantage of returning the protein to a defined lipid environment, and the choice of system depends on the application. Regardless of the system to be used, the fundamental process involves the removal of detergent and incorporation of the protein into a stable lipid system. This chapter details methodologies we have developed, mainly focussed on the model G protein-coupled receptor (GPCR) neurotensin receptor 1, and the GPCR-homologue and model, bacteriorhopdopsin.

Publication DOI: https://doi.org/10.1016/bs.mie.2015.01.004
Divisions: Life & Health Sciences
Additional Information: © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords: bacteriorhopdopsin,cell membrane,GPCR-homologue,lipid structure G protein-coupled receptor,membrane proteins,neurotensin receptor 1,Biochemistry,Molecular Biology,Medicine(all)
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2015
Authors: Goddard, Alan D. ( 0000-0003-4950-7470)
Dijkman, Patricia M.
Adamson, Roslin J.
dos Reis, Rosana Inácio
Watts, Anthony

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