Label-free oligonucleotide biosensor based on dual-peak long period fiber grating

Chen, Xianfeng, Liu, Chen, Cardoso, Marcos R., Mendonça, Cleber R., Nagel, David A., Hine, Anna V. and Zhang, Lin (2016). Label-free oligonucleotide biosensor based on dual-peak long period fiber grating. IN: Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI. Gannot, Israel (ed.) SPIE Proceedings . Bellingham, WA (US): SPIE.

Abstract

We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.

Publication DOI: https://doi.org/10.1117/12.2207869
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Engineering & Applied Sciences > Electrical, electronic & power engineering
Engineering & Applied Sciences > Institute of Photonics
Additional Information: Xianfeng Chen ; Chen Liu ; Marcos R. Cardoso ; Cleber R. Mendonça ; David A. Nagel ; Anna V. Hine and Lin Zhang; "Label-free oligonucleotide biosensor based on dual-peak long period fiber grating", Proc. SPIE 9702, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVI, 97020S (March 7, 2016). Copyright 2016. Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2207869
Event Title: SPIE Photonics West BiOS 2016
Event Type: Other
Event Location: The Moscone Center
Event Dates: 2016-02-13 - 2016-02-18
Uncontrolled Keywords: DNA,EDC-mediated reaction,label-free,long period grating,oligonucleotide,optical fiber biosensor,Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials,Biomaterials,Radiology Nuclear Medicine and imaging
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Related URLs: http://www.scopus.com/inward/record.url?scp=84973338417&partnerID=8YFLogxK (Scopus URL)
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2501733 (Publisher URL)
Published Date: 2016-03-07
Authors: Chen, Xianfeng
Liu, Chen
Cardoso, Marcos R.
Mendonça, Cleber R.
Nagel, David A.
Hine, Anna V. ( 0000-0003-4065-831X)
Zhang, Lin ( 0000-0002-1691-5577)

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