c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE

Goddard, Alan D., Stevens, Julie M., Rao, Feng, Mavridou, Despoina A.I., Chan, Weelee, Richardson, David J., Allen, James W.A. and Ferguson, Stuart J. (2010). c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 285 (30), pp. 22882-22889.

Abstract

The Ccm cytochrome c maturation System I catalyzes covalent attachment of heme to apocytochromes c in many bacterial species and some mitochondria. A covalent, but transient, bond between heme and a conserved histidine in CcmE along with an interaction between CcmH and the apocytochrome have been previously indicated as core aspects of the Ccm system. Here, we show that in the Ccm system from Desulfovibrio desulfuricans, no CcmH is required, and the holo-CcmE covalent bond occurs via a cysteine residue. These observations call for reconsideration of the accepted models of System I-mediated c-type cytochrome biogenesis.

Publication DOI: https://doi.org/10.1074/jbc.M110.133421
Divisions: Life & Health Sciences
Additional Information: © 2010 by The American Society for Biochemistry and Molecular Biology, Inc. Errata: Goddard, A. D., Stevens, J. M., Rao, F., Mavridou, D. A. I., Chan, W., Richardson, D. J., ... Ferguson, S. J. (2011). c-Type cytochrome biogenesis can occur via a natural Ccm system lacking CcmH, CcmG, and the heme-binding histidine of CcmE. Journal of Biological Chemistry, 286(33), 29442. DOI: 10.1074/jbc.A110.133421
Uncontrolled Keywords: chaperone chaperonin,cytochrome c,cytochromes,heme,post-translational modification,protein assembly,cytochrome c biogenesis,cytochrome c maturation ,heme chaperone,Biochemistry,Cell Biology,Molecular Biology
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2010-07-23
Authors: Goddard, Alan D. ( 0000-0003-4950-7470)
Stevens, Julie M.
Rao, Feng
Mavridou, Despoina A.I.
Chan, Weelee
Richardson, David J.
Allen, James W.A.
Ferguson, Stuart J.

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