Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration

Luo, Binbin, Yan, Zhijun, Sun, Zhongyuan, Liu, Yong, Zhao, Mingfu and Zhang, Lin (2015). Biosensor based on excessively tilted fiber grating in thin-cladding optical fiber for sensitive and selective detection of low glucose concentration. Optics Express, 23 (25), pp. 32429-32440.

Abstract

We report a highly sensitive, high Q-factor, label free and selective glucose sensor by using excessively tilted fiber grating (Ex-TFG) inscribed in the thin-cladding optical fiber (TCOF). Glucose oxidase (GOD) was covalently immobilized on optical fiber surface and the effectiveness of GOD immobilization was investigated by the fluorescence microscopy and highly accurate spectral interrogation method. In contrast to the long period grating (LPG) and optical fiber (OF) surface Plasmon resonance (SPR) based glucose sensors, the Ex-TFG configuration has merits of nearly independent cross sensitivity of the environmental temperature, simple fabrication method (no noble metal deposition or cladding etching) and high detection accuracy (or Q-factor). Our experimental results have shown that Ex-TFG in TCOF based sensor has a reliable and fast detection for the glucose concentration as low as 0.1~2.5mg/ml and a high sensitivity of ~1.514nm·(mg/ml)−1, which the detection accuracy is ~0.2857nm−1 at pH 5.2, and the limit of detection (LOD) is 0.013~0.02mg/ml at the pH range of 5.2~7.4 by using an optical spectrum analyzer with a resolution of 0.02nm.

Publication DOI: https://doi.org/10.1364/OE.23.032429
Divisions: Engineering & Applied Sciences > Institute of Photonics
Engineering & Applied Sciences > Electrical, electronic & power engineering
Uncontrolled Keywords: Atomic and Molecular Physics, and Optics
Full Text Link: https://www.osapublishing.org/abstract.cfm?URI=oe-23-25-32429
Related URLs: http://www.scopus.com/inward/record.url?scp=84959387628&partnerID=8YFLogxK (Scopus URL)
Published Date: 2015-12-09
Authors: Luo, Binbin
Yan, Zhijun
Sun, Zhongyuan
Liu, Yong
Zhao, Mingfu
Zhang, Lin ( 0000-0002-1691-5577)

Export / Share Citation


Statistics

Additional statistics for this record