Optimising Pichia pastoris induction

Bawa, Zharain and Darby, Richard A.J. Optimising Pichia pastoris induction. IN: Recombinant protein production in yeast. Bill, Roslyn M. (ed.) Springer protocols . Humana Press.


A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be controlled and monitored throughout the process in order to maximise recombinant protein yields. Described here are online and off-line methods to monitor and control methanol addition to bench-top-scale bioreactors.

Publication DOI: https://doi.org/10.1007/978-1-61779-770-5_15
Divisions: Life & Health Sciences > Biosciences
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Uncontrolled Keywords: methanol,mixed feeds,optimisation,yeast,Molecular Biology,Genetics


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