Large-scale production of secreted proteins in Pichia pastoris

Bora, Nagamani (2012). Large-scale production of secreted proteins in Pichia pastoris. IN: Recombinant protein production in yeast. Bill, Roslyn M. (ed.) Springer protocols . Humana Press.

Abstract

The production of recombinant therapeutic proteins is an active area of research in drug development. These bio-therapeutic drugs target nearly 150 disease states and promise to bring better treatments to patients. However, if new bio-therapeutics are to be made more accessible and affordable, improvements in production performance and optimization of processes are necessary. A major challenge lies in controlling the effect of process conditions on production of intact functional proteins. To achieve this, improved tools are needed for bio-processing. For example, implementation of process modeling and high-throughput technologies can be used to achieve quality by design, leading to improvements in productivity. Commercially, the most sought after targets are secreted proteins due to the ease of handling in downstream procedures. This chapter outlines different approaches for production and optimization of secreted proteins in the host Pichia pastoris.

Publication DOI: https://doi.org/10.1007/978-1-61779-770-5_19
Divisions: Life & Health Sciences > Biosciences
Uncontrolled Keywords: bioreactor,induction,Pichia pastoris,promoter,secreted protein,vector,Molecular Biology,Genetics
Published Date: 2012-02-25

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