CD4+ T cell surface alpha enolase is lower in older adults

Bennett, Stuart J., Augustyniak, Edyta M., Dunston, Christopher R., Brown, Richard A., Shantsila, Eduard, Lip, Gregory Y.H., Torrão, Rita D.C., Pararasa, Chathyan, Hussein, Ali R., Ladouce, Romain, Friguet, Bertrand and Griffiths, Helen R. (2015). CD4+ T cell surface alpha enolase is lower in older adults. Mechanisms of Ageing and Development, 152 , 56–62.

Abstract

To identify novel cell ageing markers in order to gain insight into ageing mechanisms, we adopted membrane enrichment and comparison of the CD4+ T cell membrane proteome (purified by cell surface labelling using Sulfo-NHS-SS-Biotin reagent) between healthy young (n=9, 20-25y) and older (n=10; 50-70y) male adults. Following two-dimensional gel electrophoresis (2DE) to separate pooled membrane proteins in triplicates, the identity of protein spots with age-dependent differences (p<0.05 and >1.4 fold difference) was determined using liquid chromatography-mass spectrometry (LC-MS/MS). Seventeen protein spot density differences (ten increased and seven decreased in the older adult group) were observed between young and older adults. From spot intensity analysis, CD4+ T cell surface α-enolase was decreased in expression by 1.5 fold in the older age group; this was verified by flow cytometry (n=22) and qPCR with significantly lower expression of cellular α-enolase mRNA and protein compared to young adult CD4+ T cells (p<0.05). In an independent age-matched case-control study, lower CD4+ T cell surface α-enolase expression was observed in age-matched patients with cardiovascular disease (p<0.05). An immune-modulatory role has been proposed for surface α-enolase and our findings of decreased expression suggest that deficits in surface α-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease.

Publication DOI: https://doi.org/10.1016/j.mad.2015.09.005
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences > Pharmacy
Life & Health Sciences
Additional Information: © 2015, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Funding: MARK-AGE (EU FP7 Large-scale integrating Project HEALTH-F4-2008-200880) and COST CM1001. Supplementary data avaialbe on the jornal website.
Uncontrolled Keywords: enolase,phenotype,2D gel electrophoresis,CD4+T cell,plasma membrane proteomics,Ageing,Developmental Biology
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2015-12
Authors: Bennett, Stuart J.
Augustyniak, Edyta M.
Dunston, Christopher R.
Brown, Richard A.
Shantsila, Eduard
Lip, Gregory Y.H.
Torrão, Rita D.C.
Pararasa, Chathyan
Hussein, Ali R.
Ladouce, Romain
Friguet, Bertrand
Griffiths, Helen R. ( 0000-0002-2666-2147)

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