Amyloid β 1-42 induces hypometabolism in human stem cell-derived neuron and astrocyte networks

Tarczyluk, Marta A., Nagel, David A., Parri, H. Rhein, Tse, Erin H.Y., Brown, James E., Coleman, Michael D. and Hill, Eric J. (2015). Amyloid β 1-42 induces hypometabolism in human stem cell-derived neuron and astrocyte networks. Journal of Cerebral Bood Fow and Metabolism, 35 (8), pp. 1348-1357.

Abstract

Alzheimer's disease (AD) is the most common form of dementia, affecting more than 35 million people worldwide. Brain hypometabolism is a major feature of AD, appearing decades before cognitive decline and pathologic lesions. To date, the majority of studies on hypometabolism in AD have used transgenic animal models or imaging studies of the human brain. As it is almost impossible to validate these findings using human tissue, alternative models are required. In this study, we show that human stem cell-derived neuron and astrocyte cultures treated with oligomers of amyloid beta 1-42 (Aβ1-42) also display a clear hypometabolism, particularly with regard to utilization of substrates such as glucose, pyruvate, lactate, and glutamate. In addition, a significant increase in the glycogen content of cells was also observed. These changes were accompanied by changes in NAD+ /NADH, ATP, and glutathione levels, suggesting a disruption in the energy-redox axis within these cultures. The high energy demands associated with neuronal functions such as memory formation and protection from oxidative stress put these cells at particular risk from Aβ-induced hypometabolism. Further research using this model may elucidate the mechanisms associated with Aβ-induced hypometabolism.

Publication DOI: https://doi.org/10.1038/jcbfm.2015.58
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Life & Health Sciences > Pharmacy
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Funding: Alzheimer's Research UK (PPG2009B-3). Supplementary available on the journal website.
Uncontrolled Keywords: Alzheimer's disease,amyloid,astrocytes,metabolism,neurons,stem cells,Cardiology and Cardiovascular Medicine,Clinical Neurology,Neurology
Full Text Link: http://www.natu ... bfm201558a.html
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2015-08-01
Authors: Tarczyluk, Marta A.
Nagel, David A.
Parri, H. Rhein ( 0000-0002-1412-2688)
Tse, Erin H.Y.
Brown, James E. ( 0000-0002-3504-7373)
Coleman, Michael D.
Hill, Eric J. ( 0000-0002-9419-1500)

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