G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent

Jamshad, Mohammed, Charlton, Jack, Lin, Yu-Pin, Routledge, Sarah J, Bawa, Zharain, Knowles, Timothy J., Overduin, Michael, Dekker, Niek, Dafforn, Tim R., Bill, Roslyn M., Poyner, David R. and Wheatley, Mark (2015). G-protein-coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Bioscience Reports, 35 (2),

Abstract

G-protein coupled receptors (GPCRs) constitute the largest class of membrane proteins and are a major drug target. A serious obstacle to studying GPCR structure/function characteristics is the requirement to extract the receptors from their native environment in the plasma membrane, coupled with the inherent instability of GPCRs in the detergents required for their solubilization. In the present study, we report the first solubilization and purification of a functional GPCR [human adenosine A<inf>2A</inf> receptor (A<inf>2A</inf>R)], in the total absence of detergent at any stage, by exploiting spontaneous encapsulation by styrene maleic acid (SMA) co-polymer direct from the membrane into a nanoscale SMA lipid particle (SMALP). Furthermore, the A<inf>2A</inf>R-SMALP, generated from yeast (Pichia pastoris) or mammalian cells, exhibited increased thermostability (∼5°C) compared with detergent [DDM (n-dodecyl-β-D-maltopyranoside)]-solubilized A<inf>2A</inf>R controls. The A<inf>2A</inf>R-SMALP was also stable when stored for prolonged periods at 4°C and was resistant to multiple freeze-thaw cycles, in marked contrast with the detergent-solubilized receptor. These properties establish the potential for using GPCR-SMALP in receptor-based drug discovery assays. Moreover, in contrast with nanodiscs stabilized by scaffold proteins, the non-proteinaceous nature of the SMA polymer allowed unobscured biophysical characterization of the embedded receptor. Consequently, CD spectroscopy was used to relate changes in secondary structure to loss of ligand binding ([<sup>3</sup>H]ZM241385) capability. SMALP-solubilization of GPCRs, retaining the annular lipid environment, will enable a wide range of therapeutic targets to be prepared in native-like state to aid drug discovery and understanding of GPCR molecular mechanisms.

Publication DOI: https://doi.org/10.1042/BSR20140171
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Pharmacy
Additional Information: ©2015 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. Funding: BBSRC (BB/I020349/1; BB/I019960/1; BB/J010812/1; BB/J017310/1; and MRC/Astra Zeneca (G1001610).
Uncontrolled Keywords: adenosine receptor,detergent-free,g-protein coupled receptor (GPCR),protein thermostability,structure,Biochemistry,Biophysics,Cell Biology,Molecular Biology
Full Text Link: http://www.bioscirep.org/bsr/035/bsr035e188.htm
Related URLs: http://www.scopus.com/inward/record.url?scp=84927919762&partnerID=8YFLogxK (Scopus URL)
Published Date: 2015-02-27
Authors: Jamshad, Mohammed
Charlton, Jack
Lin, Yu-Pin
Routledge, Sarah J
Bawa, Zharain
Knowles, Timothy J.
Overduin, Michael
Dekker, Niek
Dafforn, Tim R.
Bill, Roslyn M. ( 0000-0003-1331-0852)
Poyner, David R. ( 0000-0003-1590-112X)
Wheatley, Mark

Download

[img]

Version: Published Version

License: Creative Commons Attribution


Export / Share Citation


Statistics

Additional statistics for this record