Representational difference analysis:Critical appraisal and method development for the identification of unique DNA sequences from prokaryotes

Allen, Natalie L., Penn, Charles W. and Hilton, Anthony C. (2003). Representational difference analysis:Critical appraisal and method development for the identification of unique DNA sequences from prokaryotes. Journal of Microbiological Methods, 55 (1), pp. 73-81.

Abstract

Representational difference analysis (RDA) has great potential for preferential amplification of unique but uncharacterised DNA sequences present in one source such as a whole genome, but absent from a related genome or other complex population of sequences. While a few examples of its successful exploitation have been published, the method has not been well dissected and robust, detailed published protocols are lacking. Here we examine the method in detail, suggest improvements and provide a protocol that has yielded key unique sequences from a pathogenic bacterial genome. © 2003 Elsevier Science B.V. All rights reserved.

Publication DOI: https://doi.org/10.1016/S0167-7012(03)00117-9
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: driver,kinetic enrichment,RDA,representational difference analysis,subtractive hybridization,tester,Biotechnology,Applied Microbiology and Biotechnology,Microbiology
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2003-10
Authors: Allen, Natalie L.
Penn, Charles W.
Hilton, Anthony C. ( 0000-0001-8025-5270)

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