Glucosamine-induced insulin resistance in L6 muscle cells

Bailey, C.J. and Turner, S.L. (2004). Glucosamine-induced insulin resistance in L6 muscle cells. Diabetes, Obesity and Metabolism, 6 (4), pp. 293-298.


Background: Glucosamine increases flux through the hexosamine pathway, causing insulin resistance and disturbances similar to diabetic glucose toxicity. Aim: This study examines the effect of glucosamine on glucose uptake by cultured L6 muscle cells as a model of insulin resistance. Methods: Glucose uptake by L6 myotubes was measured using the non-metabolized glucose analogue 2-deoxy-D-glucose after incubation with glucosamine for 4 and 24 h, with and without insulin and several other agents (metformin, peroxovanadium and D-pinitol) that improve glucose uptake in diabetic states. Results: After 4 h, high concentrations of glucosamine (5 × 10-3 and 10-2 M) reduced basal and insulin-stimulated glucose uptake by up to 50%. After 24 h, the effect of insulin was completely abolished by 10-2 M glucosamine and reduced over 50% by 5 × 10-3 M glucosamine. Lower concentrations of glucosamine did not significantly alter glucose uptake. The effect of glucosamine could not be attributed to cytotoxicity assessed by the Trypan Blue test. Metformin, peroxovanadium and D-pinitol, each of which increased glucose uptake by L6 cells, did not prevent the decrease in glucose uptake with glucosamine. Conclusion: Glucosamine decreased insulin-stimulated glucose uptake by L6 muscle cells, providing a potential model of insulin resistance with similarities to glucose toxicity. Insulin resistance induced by glucosamine was not reversed by three agents (metformin, peroxovanadium and D-pinitol) known to enhance or partially mimic the effects of insulin. © 2004 Blackwell Publishing Ltd.

Publication DOI:
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: glucosamine,glucose toxicity,insulin resistance,metformin,muscle cells,peroxovanadium,pinitol,Internal Medicine,Endocrinology,Endocrinology, Diabetes and Metabolism
Full Text Link: http://onlineli ... 0350.x/abstract
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2004-07
Authors: Bailey, C.J. ( 0000-0002-6998-6811)
Turner, S.L.

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