The application of counter immunoelectrophoresis (CIE) in ocular protein studies. Part I:time dependent deposition patterns of immunoregulatory proteins on anionic hydrogel contact lenses

Mann, Aisling M., Jones, Lyndon W. and Tighe, Brian J. (2002). The application of counter immunoelectrophoresis (CIE) in ocular protein studies. Part I:time dependent deposition patterns of immunoregulatory proteins on anionic hydrogel contact lenses. Contact Lens and Anterior Eye, 25 (2), pp. 73-80.

Abstract

This paper focuses on the effects of wear regime on the deposition pattern of important immunoregulatory proteins on FDA Group IV etafilcon-A lenses. Specifically, the aim was to assess the extent to which the daily disposable wear modality produces a different deposition of proteins from the conventional daily wear regime which is coupled with cleaning and disinfection. Counter immunoelectrophoresis (CIE) was employed to detect individual proteins in lens extracts from individual patients and focused on the analysis of five proteins, IgA, IgG, lactoferrin, albumin and kininogen. Deposition was monitored as a function of time; significantly lower deposition was detected on the daily disposable lenses. cr 2002 British Contact Lens Association. Published by Elsevier Science Ltd. All rights reserved.

Publication DOI: https://doi.org/10.1016/S1367-0484(02)00003-6
Divisions: Engineering & Applied Sciences > Chemical engineering & applied chemistry
Engineering & Applied Sciences > Polymer and advanced materials research group
Additional Information: Copyright 2008 Elsevier B.V., All rights reserved.
Uncontrolled Keywords: protein deposition,counter immunoelectrophoresis,patient comfort,time dependency,biocompatibility
Full Text Link:
Related URLs: http://www.scopus.com/inward/record.url?scp=0036330581&partnerID=8YFLogxK (Scopus URL)
Published Date: 2002-06
Authors: Mann, Aisling M.
Jones, Lyndon W.
Tighe, Brian J.

Download

Item under embargo.

Export / Share Citation


Statistics

Additional statistics for this record