Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets

Brown, James E.P., Thomas, Steven, Digby, Janet E. and Dunmore, Simon J. (2002). Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets. FEBS Letters, 513 (2-3), pp. 189-192.

Abstract

Elevated islet uncoupling protein-2 (UCP-2) impairs β-cell function and UCP-2 may be increased in clinical obesity and diabetes. We investigated the effects of glucose and leptin on UCP-2 expression in isolated human islets. Human islets were incubated for 24 h with glucose (5.5–22 mmol/l)±leptin (0–10 nmol/l). Some islet batches were incubated at high (22 mmol/l), and subsequently lower (5.5 mmol/l), glucose to assess reversibility of effects. Leptin effects on insulin release were also measured. Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. This was not fully reversed by subsequently reduced glucose levels. Leptin decreased UCP-2 expression by up to 75%, and maximally inhibited insulin release by 47%, at 22 mmol/l glucose. This is the first report of UCP-2 expression in human islets and provides novel evidence of its role in the loss of β-cell function in diabetes.

Publication DOI: https://doi.org/10.1016/S0014-5793(02)02296-2
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Additional Information: Open Archive
Uncontrolled Keywords: β-Cell,gene expression,glucose toxicity,type 2 diabetes
Published Date: 2002-02-27
Authors: Brown, James E.P. ( 0000-0002-3504-7373)
Thomas, Steven
Digby, Janet E.
Dunmore, Simon J.

Download

Item under embargo.

Export / Share Citation


Statistics

Additional statistics for this record