Macrophage-mediated clearance of cells undergoing caspase-3-independent death

Turner, C., Devitt, A., Parker, K., MacFarlane, M., Giuliano, M., Cohen, G.M. and Gregory, C.D. (2003). Macrophage-mediated clearance of cells undergoing caspase-3-independent death. Cell Death and Differentiation, 10 (3), pp. 302-312.

Abstract

Little is known of the functions of caspases in mediating the surface changes required for phagocytosis of dying cells. Here we investigate the role played by the effector caspase, caspase-3 in this process using the caspase-3-defective MCF-7 breast carcinoma line and derived caspase-3-expressing transfectants. Our results indicate that, while certain typical features of apoptosis induced by etoposide – namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments – are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages – both by CD14-dependent and CD14-independent mechanisms – do not require caspase-3. Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. These results demonstrate directly that cell surface changes that are sufficient for anti-inflammatory clearance by human macrophages can be regulated independently of stereotypical features of the apoptosis programme that require caspase-3.

Publication DOI: https://doi.org/10.1038/sj.cdd.4401170
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: caspases,monocytes/macrophages,cell surface,CD14,phosphatidylserine,inflammation,phagocytosis
Full Text Link: http://www.natu ... l/4401170a.html
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Published Date: 2003-03
Authors: Turner, C.
Devitt, A. ( 0000-0002-4651-6761)
Parker, K.
MacFarlane, M.
Giuliano, M.
Cohen, G.M.
Gregory, C.D.

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