Macrophage-mediated clearance of cells undergoing caspase-3-independent death

Turner, C., Devitt, A., Parker, K., MacFarlane, M., Giuliano, M., Cohen, G.M. and Gregory, C.D. (2003). Macrophage-mediated clearance of cells undergoing caspase-3-independent death. Cell Death and Differentiation, 10 (3), pp. 302-312.


Little is known of the functions of caspases in mediating the surface changes required for phagocytosis of dying cells. Here we investigate the role played by the effector caspase, caspase-3 in this process using the caspase-3-defective MCF-7 breast carcinoma line and derived caspase-3-expressing transfectants. Our results indicate that, while certain typical features of apoptosis induced by etoposide – namely classical morphological changes and the ability to degrade DNA into oligonucleosomal fragments – are caspase-3-dependent, loss of cell adhesion to plastic and the capacity to interact with, and to be phagocytosed by, human monocyte-derived macrophages – both by CD14-dependent and CD14-independent mechanisms – do not require caspase-3. Furthermore, both etoposide-induced caspase-3-positive and -negative MCF-7 cells suppressed proinflammatory cytokine release by macrophages. These results demonstrate directly that cell surface changes that are sufficient for anti-inflammatory clearance by human macrophages can be regulated independently of stereotypical features of the apoptosis programme that require caspase-3.

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Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: caspases,monocytes/macrophages,cell surface,CD14,phosphatidylserine,inflammation,phagocytosis
Full Text Link: http://www.natu ... l/4401170a.html
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Published Date: 2003-03
Authors: Turner, C.
Devitt, A. ( 0000-0002-4651-6761)
Parker, K.
MacFarlane, M.
Giuliano, M.
Cohen, G.M.
Gregory, C.D.

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