High-throughput manufacturing of size-tuned liposomes by a new microfluidics method using enhanced statistical tools for characterization

Kastner, Elisabeth, Kaur, Randip, Lowry, Deborah, Moghaddam, Behfar, Wilkinson, Alexander and Perrie, Yvonne (2014). High-throughput manufacturing of size-tuned liposomes by a new microfluidics method using enhanced statistical tools for characterization. International Journal of Pharmaceutics, 477 (1-2), pp. 361-368.

Abstract

Microfluidics has recently emerged as a new method of manufacturing liposomes, which allows for reproducible mixing in miliseconds on the nanoliter scale. Here we investigate microfluidics-based manufacturing of liposomes. The aim of these studies was to assess the parameters in a microfluidic process by varying the total flow rate (TFR) and the flow rate ratio (FRR) of the solvent and aqueous phases. Design of experiment and multivariate data analysis were used for increased process understanding and development of predictive and correlative models. High FRR lead to the bottom-up synthesis of liposomes, with a strong correlation with vesicle size, demonstrating the ability to in-process control liposomes size; the resulting liposome size correlated with the FRR in the microfluidics process, with liposomes of 50 nm being reproducibly manufactured. Furthermore, we demonstrate the potential of a high throughput manufacturing of liposomes using microfluidics with a four-fold increase in the volumetric flow rate, maintaining liposome characteristics. The efficacy of these liposomes was demonstrated in transfection studies and was modelled using predictive modeling. Mathematical modelling identified FRR as the key variable in the microfluidic process, with the highest impact on liposome size, polydispersity and transfection efficiency. This study demonstrates microfluidics as a robust and high-throughput method for the scalable and highly reproducible manufacture of size-controlled liposomes. Furthermore, the application of statistically based process control increases understanding and allows for the generation of a design-space for controlled particle characteristics.

Publication DOI: https://doi.org/10.1016/j.ijpharm.2014.10.030
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Additional Information: © 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) Funding: EPSRC; NewTBVAC (contract no. HEALTHF3-2009-241745)and Aston University Supplementary data at http://dx.doi.org/10.1016/j.ijpharm.2014.10.030
Uncontrolled Keywords: design of experiment,DNA delivery,high-throughput manufacturing,liposomes,microfluidics,Pharmaceutical Science
Full Text Link:
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2014-12-30
Authors: Kastner, Elisabeth
Kaur, Randip
Lowry, Deborah
Moghaddam, Behfar
Wilkinson, Alexander
Perrie, Yvonne

Download

[img]

Version: Published Version

License: Creative Commons Attribution


Export / Share Citation


Statistics

Additional statistics for this record