Analysis of protein carbonylation - pitfalls and promise in commonly used methods

Abstract

Abstract Oxidation of proteins has received a lot of attention in the last decades due to the fact that they have been shown to accumulate and to be implicated in the progression and the patho-physiology of several diseases such as Alzheimer, coronary heart diseases, etc. This has also resulted in the fact that research scientist became more eager to be able to measure accurately the level of oxidized protein in biological materials, and to determine the precise site of the oxidative attack on the protein, in order to get insights into the molecular mechanisms involved in the progression of diseases. Several methods for measuring protein carbonylation have been implemented in different laboratories around the world. However, to date no methods prevail as the most accurate, reliable and robust. The present paper aims at giving an overview of the common methods used to determine protein carbonylation in biological material as well as to highlight the limitations and the potential. The ultimate goal is to give quick tips for a rapid decision making when a method has to be selected and taking into consideration the advantage and drawback of the methods.

Publication DOI: https://doi.org/10.3109/10715762.2014.944868
Divisions: College of Health & Life Sciences
College of Health & Life Sciences > Chronic and Communicable Conditions
College of Health & Life Sciences > School of Biosciences > Cell & Tissue Biomedical Research
Additional Information: Copyright © 2014, Informa Healthcare
Uncontrolled Keywords: carbonylation,standardization,mass spectrometry,derivatization,immunoaffinity
Publication ISSN: 1029-2470
Last Modified: 08 Mar 2024 08:09
Date Deposited: 11 Aug 2014 10:45
Full Text Link:
Related URLs: http://informah ... 762.2014.944868 (Publisher URL)
PURE Output Type: Article
Published Date: 2014
Published Online Date: 2014-07-29
Authors: Rogowska-Wrzesinska, A.
Wojdyla, K.
Nedić, O.
Baron, C.P.
Griffiths, H.R. (ORCID Profile 0000-0002-2666-2147)

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