Vitamin C supplementation in normal subjects reduces constitutive ICAM-1 expression

Rayment, Sarah J., Shaw, Jacqui, Woollard, Kevin J., Lunec, Joe and Griffiths, Helen R. (2003). Vitamin C supplementation in normal subjects reduces constitutive ICAM-1 expression. Biochemical and Biophysical Research Communications, 308 (2), pp. 339-345.


Regulation of monocyte adhesion molecule gene expression is via redox sensitive transcription factors. We have investigated whether dietary antioxidant supplementation with vitamin C (250mg/day) can modulate monocyte ICAM-1 expression in healthy male subjects with low plasma vitamin C at baseline. In a randomised, double-blind, crossover study, monocyte ICAM-1 mRNA was analysed using quantitative reverse transcriptase PCR. Protein was determined by flow cytometry (monocytes) and ELISA (plasma). Monocyte numbers were unaltered by supplementation. Subjects with low plasma vitamin C (<50μM) prior to supplementation expressed higher levels of monocyte ICAM-1mRNA, and showed a significant (50%) reduction in ICAM-1mRNA expression after 6 weeks of 250mg/day vitamin C supplementation (p<0.05). This was paralleled by a reduction in sICAM-1 (p<0.05). For the first time, these results show that dietary vitamin C can modulate monocyte ICAM-1 gene expression in vivo, where regulation of gene expression represents a novel mechanism for benefit from dietary antioxidants. © 2003 Elsevier Inc. All rights reserved.

Publication DOI:
Divisions: Life & Health Sciences
Uncontrolled Keywords: gene expression,ICAM-1,monocytes,vitamin C,Biochemistry,Biophysics,Molecular Biology
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Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2003-08-22
Authors: Rayment, Sarah J.
Shaw, Jacqui
Woollard, Kevin J.
Lunec, Joe
Griffiths, Helen R. ( 0000-0002-2666-2147)


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