Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements

Mittal, Saloni, Revell, Matthew, Barone, Francesca, Hardie, Debbie L., Matharu, Gulraj S., Davenport, Alison J., Martin, Richard A., Grant, Melissa, Mosselmans, Frederick, Pynsent, Paul, Sumathi, Vaiyapuri P., Addison, Owen, Revell, Peter A. and Buckley, Christopher D. (2013). Lymphoid aggregates that resemble tertiary lymphoid organs define a specific pathological subset in metal-on-metal hip replacements. PLoS ONE, 8 (5),

Abstract

Aseptic lymphocyte-dominated vasculitis-associated lesion (ALVAL) has been used to describe the histological lesion associated with metal-on-metal (M-M) bearings. We tested the hypothesis that the lymphoid aggregates, associated with ALVAL lesions resemble tertiary lymphoid organs (TLOs). Histopathological changes were examined in the periprosthetic tissue of 62 M-M hip replacements requiring revision surgery, with particular emphasis on the characteristics and pattern of the lymphocytic infiltrate. Immunofluorescence and immunohistochemistry were used to study the classical features of TLOs in cases where large organized lymphoid follicles were present. Synchrotron X-ray fluorescence (XRF) measurements were undertaken to detect localisation of implant derived ions/particles within the samples. Based on type of lymphocytic infiltrates, three different categories were recognised; diffuse aggregates (51%), T cell aggregates (20%), and organised lymphoid aggregates (29%). Further investigation of tissues with organised lymphoid aggregates showed that these tissues recapitulate many of the features of TLOs with T cells and B cells organised into discrete areas, the presence of follicular dendritic cells, acquisition of high endothelial venule like phenotype by blood vessels, expression of lymphoid chemokines and the presence of plasma cells. Co-localisation of implant-derived metals with lymphoid aggregates was observed. These findings suggest that in addition to the well described general foreign body reaction mediated by macrophages and a T cell mediated type IV hypersensitivity response, an under-recognized immunological reaction to metal wear debris involving B cells and the formation of tertiary lymphoid organs occurs in a distinct subset of patients with M-M implants.

Publication DOI: https://doi.org/10.1371/journal.pone.0063470
Divisions: Life & Health Sciences > Biosciences
Additional Information: © 2013 Mittal et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Full Text Link: http://www.plos ... al.pone.0063470
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2013-05-28
Authors: Mittal, Saloni
Revell, Matthew
Barone, Francesca
Hardie, Debbie L.
Matharu, Gulraj S.
Davenport, Alison J.
Martin, Richard A. ( 0000-0002-6013-2334)
Grant, Melissa
Mosselmans, Frederick
Pynsent, Paul
Sumathi, Vaiyapuri P.
Addison, Owen
Revell, Peter A.
Buckley, Christopher D.

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