Introduction of structural diversity into oligonucleotides containing 6-thioguanine via on-column conjugation

Zheng, Qinguo, Wang, Yang and Lattmann, Eric (2003). Introduction of structural diversity into oligonucleotides containing 6-thioguanine via on-column conjugation. Tetrahedron, 59 (11), pp. 1925-1932.

Abstract

A method is described for the introduction of structural diversity into the thiocarbonyl group of 6-thioguanine within support-bound, fully protected oligonucleotides via 'on-column′ conjugation. 2′-Deoxy-6-thioguanosine with a chemically-labile trigger at its 6-thio function was incorporated at defined sites into chemically synthesized oligonucleotides. Following selective removal of the thio-protection group the support-immobilized oligonucleotides were conjugated with various groups on-column, and then deprotected and purified to produce a number of oligomers each containing a different modified base. Since the modification is accomplished on-column without affecting other functional and protecting groups in the oligomers this method is compatible with introducing structural diversity at multiple sites in DNA. © 2003 Elsevier Science Ltd. All rights reserved.

Publication DOI: https://doi.org/10.1016/S0040-4020(03)00147-9
Divisions: Life & Health Sciences > Pharmacy
Life & Health Sciences
Uncontrolled Keywords: DNA synthesis,modified base,modified DNA,post-synthetic modification,thioguanine,Biochemistry,Organic Chemistry,Drug Discovery
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Related URLs: http://www.scopus.com/inward/record.url?scp=0037430458&partnerID=8YFLogxK (Scopus URL)
Published Date: 2003-03-10
Authors: Zheng, Qinguo
Wang, Yang
Lattmann, Eric

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