Switching the mode of metabolism in the yeast Saccharomyces cerevisiae

Otterstedt, Karin, Larsson, Christer, Bill, Roslyn M., Ståhlberg, Anders, Boles, Eckhard, Hohmann, Stefan and Gustafsson, Lena (2004). Switching the mode of metabolism in the yeast Saccharomyces cerevisiae. Embo Reports, 5 (5), pp. 532-537.


The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction. © 2004 European Molecular Biology Organization.

Publication DOI: https://doi.org/10.1038/sj.embor.7400132
Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Uncontrolled Keywords: glycolysis,hexose transport,metabolism,respiration,signalling,Cell Biology,Genetics
Full Text Link: http://www.natu ... ll/7400132.html
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2004-05
Authors: Otterstedt, Karin
Larsson, Christer
Bill, Roslyn M. ( 0000-0003-1331-0852)
Ståhlberg, Anders
Boles, Eckhard
Hohmann, Stefan
Gustafsson, Lena

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