The use of site-directed mutagenesis to study GPCRs

Conner, Alex C.; Barwell, James; Poyner, David R. and Wheatley, Mark (2011). The use of site-directed mutagenesis to study GPCRs. IN: Receptor signal transduction protocols. Willars, Gary B. and Challiss, R.A. John (eds) Methods in molecular biology (3rd ed). Humana Press.

Abstract

G protein coupled receptors (GPCRs) are highly flexible and dynamic proteins, which are able to interact with diverse ligands, effectors, and regulatory proteins. Site-directed mutagenesis (SDM) is a powerful tool for providing insight into how these proteins actually work, both in its own right and when used in conjunction with information provided by other techniques such as crystallography or molecular modelling. Mutagenesis has been used to identify and characterise a myriad of functionally important residues, motifs and domains within the GPCR architecture, and to identify aspects of similarity and differences between the major families of GPCRs. This chapter presents the necessary information for undertaking informative SDM of these proteins. Whilst this is relevant to protein structure/function studies in -general, specific pitfalls and protocols suited to investigating GPCRs in particular will be highlighted.

Publication DOI: https://doi.org/10.1007/978-1-61779-126-0_5
Divisions: Life & Health Sciences > Pharmacy
Uncontrolled Keywords: cultured cells,genetic vectors,site-directed mutagenesis,mutant chimeric proteins,G-protein-coupled receptors,transfection,Medicine(all),Molecular Biology,Genetics
Published Date: 2011-05-05

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