Structural characterization of CD81-Claudin-1 hepatitis C virus receptor complexes

Bonander, Nicklas, Jamshad, Mohammed, Hu, Ke, Farquhar, Michelle J., Stamataki, Zania, Balfe, Peter, McKeating, Jane A. and Bill, Roslyn M (2011). Structural characterization of CD81-Claudin-1 hepatitis C virus receptor complexes. Biochemical Society Transactions, 39 (2), pp. 537-540.


Tetraspanins are thought to exert their biological function(s) by co-ordinating the lateral movement and trafficking of associated molecules into tetraspanin-enriched microdomains. A second four-TM (transmembrane) domain protein family, the Claudin superfamily, is the major structural component of cellular TJs (tight junctions). Although the Claudin family displays low sequence homology and appears to be evolutionarily distinct from the tetraspanins, CD81 and Claudin-1 are critical molecules defining HCV (hepatitis C virus) entry; we recently demonstrated that CD81-Claudin-1 complexes have an essential role in this process. To understand the molecular basis of CD81-Claudin-1 complex formation, we produced and purified milligram quantities of full-length CD81 and Claudin-1, alone and in complex, in both detergent and lipid contexts. Structural characterization of these purified proteins will allow us to define the mechanism(s) underlying virus-cell interactions and aid the design of therapeutic agents targeting early steps in the viral life cycle.

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Divisions: Life & Health Sciences > Biosciences
Life & Health Sciences
Life & Health Sciences > Applied Health Research Group
Life & Health Sciences > Biomedical Sciences research group
Uncontrolled Keywords: animals,CD antigens,CD81 antigens,hepacivirus,humans,membrane proteins,biological models,molecular conformation,multiprotein complexes,virus receptors,virus internalization,Claudin-1,hepatitis C virus ,oligomerization,tetraspanin,Biochemistry
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Published Date: 2011-04
Authors: Bonander, Nicklas
Jamshad, Mohammed
Hu, Ke
Farquhar, Michelle J.
Stamataki, Zania
Balfe, Peter
McKeating, Jane A.
Bill, Roslyn M ( 0000-0003-1331-0852)


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