Improving recombinant eukaryotic membrane protein yields in Pichia pastoris:the importance of codon optimization and clone selection

Öberg, Fredrik, Sjöhamn, Jennie, Conner, Matthew T., Bill, Roslyn M. and Hedfalk, Kristina (2011). Improving recombinant eukaryotic membrane protein yields in Pichia pastoris:the importance of codon optimization and clone selection. Molecular Membrane Biology, 28 (6), pp. 398-411.

Abstract

In the last 15 years, 80% of all recombinant proteins reported in the literature were produced in the bacterium, Escherichia coli, or the yeast, Pichia pastoris. Nonetheless, developing effective general strategies for producing recombinant eukaryotic membrane proteins in these organisms remains a particular challenge. Using a validated screening procedure together with accurate yield quantitation, we therefore wished to establish the critical steps contributing to high yields of recombinant eukaryotic membrane protein in P. pastoris. Whilst the use of fusion partners to generate chimeric constructs and directed mutagenesis have previously been shown to be effective in bacterial hosts, we conclude that this approach is not transferable to yeast. Rather, codon optimization and the preparation and selection of high-yielding P. pastoris clones are effective strategies for maximizing yields of human aquaporins.

Publication DOI: https://doi.org/10.3109/09687688.2011.602219
Divisions: Life & Health Sciences
Life & Health Sciences > Biosciences
Uncontrolled Keywords: aquaporins,codon,electroporation,humans,lithium chloride,pichia,recombinant proteins,genetic transformation,Cell Biology,Molecular Biology
Full Text Link: http://informah ... 688.2011.602219
Related URLs: http://www.scop ... tnerID=8YFLogxK (Scopus URL)
Published Date: 2011-09
Authors: Öberg, Fredrik
Sjöhamn, Jennie
Conner, Matthew T.
Bill, Roslyn M. ( 0000-0003-1331-0852)
Hedfalk, Kristina

Download

[img]

Version: Accepted Version


Export / Share Citation


Statistics

Additional statistics for this record