Self-assembly of trehalose molecules on a lysozyme surface: the broken glass hypothesis

Fedorov, Maxim V., Goodman, Jonathan M., Nerukh, Dmitry and Schumm, Stephan (2011). Self-assembly of trehalose molecules on a lysozyme surface: the broken glass hypothesis. Physical Chemistry Chemical Physics, 13 (6), pp. 2294-2299.

Abstract

To help understand how sugar interactions with proteins stabilise biomolecular structures, we compare the three main hypotheses for the phenomenon with the results of long molecular dynamics simulations on lysozyme in aqueous trehalose solution (0.75 M). We show that the water replacement and water entrapment hypotheses need not be mutually exclusive, because the trehalose molecules assemble in distinctive clusters on the surface of the protein. The flexibility of the protein backbone is reduced under the sugar patches supporting earlier findings that link reduced flexibility of the protein with its higher stability. The results explain the apparent contradiction between different experimental and theoretical results for trehalose effects on proteins.

Publication DOI: https://doi.org/10.1039/C0CP01705A
Divisions: Life & Health Sciences > Optometry
Engineering & Applied Sciences > Mathematics
Engineering & Applied Sciences > Systems analytics research institute (SARI)
Uncontrolled Keywords: sugar interactions,biomolecular structures,long molecular dynamics,lysozyme,aqueous trehalose solution,water entrapment hypotheses,Physical and Theoretical Chemistry,Physics and Astronomy(all)
Full Text Link: http://pubs.rsc.org/en/Content/ArticleLanding/2011/CP/c0cp01705a
Related URLs: http://www.scopus.com/inward/record.url?scp=79251586898&partnerID=8YFLogxK (Scopus URL)
Published Date: 2011-02-14
Authors: Fedorov, Maxim V.
Goodman, Jonathan M.
Nerukh, Dmitry ( 0000-0001-9005-9919)
Schumm, Stephan

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