8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus

Lunec, Joseph, Herbert, Karl E., Blount, Susan, Griffiths, Helen R. and Emery, Paul (1994). 8-Hydroxydeoxyguanosine. A marker of oxidative DNA damage in systemic lupus erythematosus. FEBS Letters, 348 (2), pp. 131-138.

Abstract

8-Hydroxydeoxyguanosine (80HDG) is a specific marker of oxidative damage to DNA. We have observed that patients with SLE (systemic lupus erythematosus), have undetectable levels of urinary 80HDG by HPLC. Further analysis by GC-MS confirmed that levels of 80HDG in SLE urine were 10(3)-fold lower than in an age- and sex-matched control group. Experiments utilising cultures of SLE and normal lymphocytes exposed to H2O2 confirmed the impaired ability of SLE lymphocytes to repair 80HDG. We subsequently observed in SLE patients that 80HDG had accumulated in low molecular weight DNA associated with circulating immune complexes. We suggest that oxygen radicals may induce pathology in SLE by maintaining the presence of an antigenic form of DNA in the circulation.

Publication DOI: https://doi.org/10.1016/0014-5793(94)00583-4
Divisions: Life & Health Sciences
Life & Health Sciences > Applied Health Research Group
Uncontrolled Keywords: systemic lupus erythematosus,rheumatoid arthritis
Published Date: 1994-07-11
Authors: Lunec, Joseph
Herbert, Karl E.
Blount, Susan
Griffiths, Helen R. ( 0000-0002-2666-2147)
Emery, Paul

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