Immunochemical detection of sequence-specific modifications to DNA induced by UV light

Herbert, Karl E., Mistry, Nalini, Griffiths, Helen R. and Lunec, Joseph (1994). Immunochemical detection of sequence-specific modifications to DNA induced by UV light. Carcinogenesis, 15 (11), pp. 2517-2521.

Abstract

Sequence specificity of antibodies to UV-damaged DNA has not been described previously. The antisera investigated here were specific for UV-modified DNA and were absolutely dependent upon the presence of thymine residues. Using a series of oligonucleotides in competition ELISA, increased inhibition was observed with increasing chain length of UV-polythymidylate. A minimum of three adjacent thymines was required for effective inhibition; alone, dimers of thymine were poor antigens. Although UV-irradiated poly(dC) was not antigenic, cytosines could partially replace thymines within the smallest effective epitope (T-T-T) with a high degree of sequence specificity, not previously described. The main epitope induced by UV was formed from adjacent thymines and either a 3' or a 5' pyrimidine.

Divisions: Life & Health Sciences
Life & Health Sciences > Applied Health Research Group
Uncontrolled Keywords: sequence specificity,antibody,UV-damaged DNA,UV-modified DNA,DNA,UV,thymine,UV-polythymidylate,antigenic,cytosine,epitope
Full Text Link: http://carcin.oxfordjournals.org/cgi/reprint/15/11/2517?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=Immunochemical+detection+of+sequence-specific+modifications+to+DNA+induced+by+UV+light&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT
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Published Date: 1994-11
Authors: Herbert, Karl E.
Mistry, Nalini
Griffiths, Helen R. ( 0000-0002-2666-2147)
Lunec, Joseph

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