Minett, William T. (1986). Cell Adhesion on Synthetic Polymer Substrates. PHD thesis, Aston University.
Abstract
This thesis investigates the role of synthetic plastic substrates in determining the adhesion and subsequent growth of anchorage dependent cells in vitro. This involved cell culture on specific substrate materials and the use of quantimet analysis, both light and scanning electron microscopy, X ray, photoelectron, spectrographic, surface chemical analysis and surface energetic measurement, in order to monitor and accurately characterise substrate properties. Attention has been paid to the relative properties of those materials which are claimed to be cell adhesive, and non adhesive respectively, and in addition to the claims that coatings of poly2hydroxyethyl methacrylate are capable of modulating the extent of cell adhesion and growth. A systematic series of commercial and purpose synthesised copolymer substrates are utilized to determine the relative role of specific chemical groups and physicochemical parameters in cell adhesion. This work coupled with the effect of gas plasma treatment on polystyrene substrates led to the proposal of a model system in which the cell adhesion characteristics of substrate materials can be predicted on the basis of fractional polarity measurements. This represents the first attempt to unify the factors affecting cell adhesion. The applications of this model system and the relevance of cell culture to the assessment of biomedical implant materials is discussed.
Publication DOI: | https://doi.org/10.48780/publications.aston.ac.uk.00014512 |
---|---|
Divisions: | College of Health & Life Sciences |
Additional Information: | Copyright © William T. Minett, 1986. William T. Minett asserts their moral right to be identified as the author of this thesis. This copy of the thesis has been supplied on condition that anyone who consults it is understood to recognise that its copyright rests with its author and that no quotation from the thesis and no information derived from it may be published without appropriate permission or acknowledgement. If you have discovered material in Aston Publications Explorer which is unlawful e.g. breaches copyright, (either yours or that of a third party) or any other law, including but not limited to those relating to patent, trademark, confidentiality, data protection, obscenity, defamation, libel, then please read our Takedown Policy and contact the service immediately. |
Institution: | Aston University |
Uncontrolled Keywords: | Cell adhesion,synthetic polymer substrates |
Last Modified: | 02 Apr 2025 11:12 |
Date Deposited: | 22 Feb 2011 11:28 |
Completed Date: | 1986-10 |
Authors: |
Minett, William T.
|