Griffiths, Helen R. and Lunec, Joseph
(1996).
The C1q binding activity of IgG is modified in vitro by reactive oxygen species: implications for rheumatoid arthritis.
FEBS Letters, 388
(2-3),
pp. 161-164.
Abstract
IgG can be denatured in vitro by reactive oxygen species (ROS). Native IgG activates the complement cascade through C1q. Using a modified ELISA, C1q binding activity of rheumatoid IgG has been compared to IgG denatured by neutrophil-derived ROS. The C1q binding activity of rheumatoid synovial fluid IgG is greater than the corresponding serum IgG (P < 0.01). Denaturation of IgG by activated polymorphs or the Fenton reaction decreased its C1q binding activity (P < 0.01). In vitro exposure of IgG to OH. and ROO. increased its interaction with C1q (P < 0.01). Hypochlorous acid had no effect. ROS-induced alteration to IgG-C1q binding activity may promote the inflammatory response in rheumatoid arthritis.
Publication DOI: | https://doi.org/10.1016/0014-5793(96)00542-X |
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Divisions: | Life & Health Sciences Life & Health Sciences > Applied Health Research Group |
Uncontrolled Keywords: | IgG,reactive oxygen species,C1q binding activity,rheumatoid arthritis |
Full Text Link: |
http://www.elsevier.com/wps/find/journaldescription.cws_home/506085/description#description |
Related URLs: | |
Published Date: | 1996-06-17 |
Authors: |
Griffiths, Helen R.
(![]() Lunec, Joseph |