The C1q binding activity of IgG is modified in vitro by reactive oxygen species: implications for rheumatoid arthritis

Griffiths, Helen R. and Lunec, Joseph (1996). The C1q binding activity of IgG is modified in vitro by reactive oxygen species: implications for rheumatoid arthritis. FEBS Letters, 388 (2-3), pp. 161-164.

Abstract

IgG can be denatured in vitro by reactive oxygen species (ROS). Native IgG activates the complement cascade through C1q. Using a modified ELISA, C1q binding activity of rheumatoid IgG has been compared to IgG denatured by neutrophil-derived ROS. The C1q binding activity of rheumatoid synovial fluid IgG is greater than the corresponding serum IgG (P < 0.01). Denaturation of IgG by activated polymorphs or the Fenton reaction decreased its C1q binding activity (P < 0.01). In vitro exposure of IgG to OH. and ROO. increased its interaction with C1q (P < 0.01). Hypochlorous acid had no effect. ROS-induced alteration to IgG-C1q binding activity may promote the inflammatory response in rheumatoid arthritis.

Publication DOI: https://doi.org/10.1016/0014-5793(96)00542-X
Divisions: Life & Health Sciences
Uncontrolled Keywords: IgG,reactive oxygen species,C1q binding activity,rheumatoid arthritis
Published Date: 1996-06-17

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