Novel repair action of vitamin C upon in vivo oxidative DNA damage

Cooke, Marcus S., Evans, Mark D., Podmore, Ian D., Herbert, Karl E., Mistry, Nalini, Mistry, Pratibha, Hickenbotham, Peter T., Hussieni, Amina, Griffiths, Helen R. and Lunec, Joseph (1998). Novel repair action of vitamin C upon in vivo oxidative DNA damage. FEBS Letters, 439 (3), pp. 363-367.

Abstract

There appears to be a paucity of data examining the effect of dietary antioxidants on levels of oxidative DNA damage in vivo, limiting evidence-based assessment of antioxidant efficacy, mechanisms and recommendation for optimal intake. We have examined levels of 8-oxo-2'-deoxyguanosine (8-oxodG) in mononuclear cell DNA, serum and urine from subjects undergoing supplementation with 500 mg/day vitamin C. Significant decreases in DNA levels of 8-oxodG were seen, correlating strongly with increases in plasma vitamin C concentration. Furthermore we established a timecourse for sequential, significant increases in serum and urinary 8-oxodG levels. These results illustrate, for the first time in humans, the kinetics of 8-oxodG removal and processing in vivo, suggesting a role for vitamin C in the regulation of DNA repair enzymes and thereby demonstrating a non-scavenging antioxidant effect.

Publication DOI: https://doi.org/10.1016/S0014-5793(98)01403-3
Divisions: Life & Health Sciences
Life & Health Sciences > Applied Health Research Group
Uncontrolled Keywords: antioxidant,vitamin C,antibody,8-Oxo-2′-deoxyguanosine,DNA repair,DNA damage
Published Date: 1998-11-20
Authors: Cooke, Marcus S.
Evans, Mark D.
Podmore, Ian D.
Herbert, Karl E.
Mistry, Nalini
Mistry, Pratibha
Hickenbotham, Peter T.
Hussieni, Amina
Griffiths, Helen R. ( 0000-0002-2666-2147)
Lunec, Joseph

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